A Primer on the Analysis of High-Throughput Sequencing Data for Detection of Plant Viruses
High Throughput Sequencing (HTS) is an important tool in diagnostic work, enabling the identification of unknown viruses within samples, and so a new freely available review article will be of interest to many of our Network members. It summarises:
- the steps involved in sample preparation
- DNA and RNA extraction for sequencing, and
- an overview of the wide range of bioinformatics tools available for HTS data analysis.
It also includes:
- a useful glossary
- a flow chart detailing the steps involved data analysis, and
- a summary of search strategies and considerations associated with these
- an Appendix listing sequence analysis solutions, setting out the pros and cons of various pipeline packages available.
You can read A Primer on the Analysis of High-Throughput Sequencing Data for Detection of Plant Viruses, by Kutnjak et al., published in the MDPI journal Microorganisms, using this link.
HTS is a huge field and this review combines and condenses a lot of information. It is a useful resource for HTS beginners and more experienced researchers alike.