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A Primer on the Analysis of High-Throughput Sequencing Data for Detection of Plant Viruses

High Throughput Sequencing (HTS) is an important tool in diagnostic work, enabling the identification of unknown viruses within samples, and so a new freely available review article will be of interest to many of our Network members. It summarises:

  • the steps involved in sample preparation
  • DNA and RNA extraction for sequencing, and
  • an overview of the wide range of bioinformatics tools available for HTS data analysis.

It also includes:

  • a useful glossary
  • a flow chart detailing the steps involved data analysis, and
  • a summary of search strategies and considerations associated with these
  • an Appendix listing sequence analysis solutions, setting out the pros and cons of various pipeline packages available.

You can read A Primer on the Analysis of High-Throughput Sequencing Data for Detection of Plant Viruses, by Kutnjak et al., published in the MDPI journal Microorganisms, using this link.

HTS is a huge field and this review combines and condenses a lot of information. It is a useful resource for HTS beginners and more experienced researchers alike.